T-DNA as an Insertional Mutagen in Arabidopsis

T-DNA as an insertional mutagen in Arabidopsis.

How effective is T-DNA insertional mutagenesis in Arabidopsis?

To evaluate the effectiveness of T-DNA insertion in knocking out a gene, 1084 published Arabidopsis thaliana insertion mutants representing 755 genes in 648 publications were reviewed. Insertion in the protein-coding region of a gene generates a knockout at least 90% of the time or 25% of the time if it is before the start codon which also produces a knockdown 67% of the time. Insertion after t...

The murine AIDS defective provirus acts as an insertional mutagen in its infected target B cells.

In susceptible mice, the murine AIDS (MAIDS) defective virus can induce marked expansion of its target cells, the majority of which belong to the B-cell lineage. This expansion, which appears to be critical for the development of the immunodeficiency syndrome, is initially polyclonal but becomes oligoclonal late in the disease, suggesting the involvement of a secondary genetic event(s) during t...

T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics

With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidoposis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool f...

T-DNA insertional mutagenesis for functional genomics in rice.

We have produced 22 090 primary transgenic rice plants that carry a T-DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel-blot and PCR analyses have shown that approximately 65% of the population contains more than one copy of the inserted T-DNA. Hygromycin resistance tests revealed that transgenic plants contain an average of 1.4 loci of T-DNA inserts. Therefore, it can ...